It wasn't a worse version or a less beautiful kind of life to live, but simply a different one that was rather stationary in comparison to my current mindset.
It is important to note that, unlike typical nucleoporins which are perceived to be rather stationary and confined within the NPC, Sec13 is dynamic and shuttles between a cytoplasmic pool and the NPC [ 42].
In what concerns the behaviour of the average q-value, we observe that for low temptation b (and low value for the cost c) the stationary q-value is rather high as an occasional defection does not cause a big loss to the cooperative partner and fast forgiveness pays off.
On the other hand, the total noise variance robust estimate in (25) is rather insensitive to the local non-stationary effects.
There is no apparent induction of 6S RNA transcription during stationary phase, however, and the observed 6S RNA accumulation is rather due to the metabolic stability of 6S RNA under stationary growth conditions.
As a consequence, the "time window" for LD isolation during cell cultivation is rather narrow and limited to the late-log/early stationary phase of growth.
The identified sources are rather localized and remain geographically stationary but show seasonally varying intensities of strong in winter and weak in summer.
The finding that SYWT and α-Syn induced cell death to a similar extent in cultures approaching stationary phase was rather surprising as it contrasted with the observations made during exponential growth where α-Syn had a more pronounced impact (Fig. 1B).
LDs isolated from yeast are rather homogeneous in size, ranging from typically 300 nm (in late log phase) to 1 μm (in stationary phase) in diameter.
