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On the other hand, when the injury is protracted for longer time, (12 weeks) cirrhosis develops and in the absence of ongoing injury there is only partial reversal with remodelling of a micro-nodular to a macro-nodular architecture [ 10].
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The most important aspect of the study is that the observation of patients was protracted for 48 h, in a clinical condition characterized by shortage of sophisticated diagnostic instruments.
Treatment will be protracted for at least 1 year after stenting (table 2).
Then PI (2.0 μg/ml medium) was added and the incubation was protracted for additional 15 min.
At the end the medium was removed, 100 μl of 50 μM H2-DCFDA were added and incubation was protracted for 30 min at 37 °C.
At the end, 20 μl of MTT solution (11 mg/ml in PBS) were added and incubation was protracted for another 2 h.
Although IE genes are the first genes to be transcribed during the infection, their function can be protracted for the entire viral replication cycle.
The incubation was protracted for 10 min and DHE-positive cells were analysed by fluorescence microscopy using an excitation wavelength of 596 nm and an emission wavelength of 620 nm.
We showed that, while CPAP treatment in acidotic ACPE patients did actually bring 50% of patients to a pH value above 7.35 within 3 (2.5 to 6) hours, the treatment nevertheless had to be protracted for at least 6 hours before the mean pH crossed the threshold of 7.35.
Care of young is protracted, extending for as long as six months, among the longest for birds.
The peak of the epidemic is protracted by about 1 day for every percent of contact reduction if this intervention starts immediately after the introduction of the infection.
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