The adoption of the model to the mouse seems to be problematic, depending on the strain used and behavioural endpoint defined.
Such a single measure, though, may be problematic depending on its composition and definition.
Reliance on nucleic acid testing alone can be problematic, depending on the target used in the assay.
The "ends-in" recombination, as in the case of chameau mutagenesis (supplementary material Fig. S12), might be problematic depending on the purpose of the genomic modifications.
In contrast, other workshop participants suggested that the use of soy-free diets could be problematic, depending on the life stage, experimental design, animal model, and end points assessed.
Even doing this to your own child could be problematic, depending on your local laws as well as your family situation.
She compares smartphone scrolling to drinking, eating, and even exercising behaviors that are fine in moderation but can become problematic depending on the person.
Notably, applying other flux prediction methods such as FBA and MOMA in this setup, given only metabolomic and proteomic data is problematic: FBA depends on a definition of a cellular objective function (commonly assumed to be the maximization of biomass yield rate in microbes), which is not available in the RBC model.
Funding of a clinical FDG-PET/MRI service may also be problematic as, depending on the relevant health-care system, if accessible at all, re-imbursement may only be available at the same level as PET/CT.
However, choosing which method to use for a particular application is problematic because its performance depends on the evaluation metric, the dataset, and so on.
