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For instance, the helices observed in ECL2 of tB1AR and hB2AR are probably constrained by the intra-ECL2 disulphide bridge and the β-strand structure observed in hAA2AR is probably constrained by a disulphide bridge between ECL1 and ECL2.
In this study system, pollen transfer is probably constrained by pollinator limitation in large patches and by mate limitation in small patches, decreasing seed production by 34% on average [ 35].
The consequences of reduced IGFBP-1 would be expected to include increased bioactivity of IGF-I and IGF-II in microenvironments where IGF bioactivity is probably constrained by IGFBP-1.
The choice of the molecular cascade, leading to drug induced – apoptosis, is probably constrained by defects in some parts of the death machinery (e.g., mutations or epigenetic repression of death receptors).
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The third and most recent eruption produced another lava flow that was largely erupted above glacial ice, but was probably constrained on its northern margin by a small glacier.
The DENV-1 evolution in Malaysia was probably constrained by purifying selection [ 52].
Maximum ω and leg angular acceleration are probably constrained by mechanical or energetic limits (Doke et al. 2005).
Additionally, NHX proteins are predicted to have ~12 transmembrane domains [ 42] and are probably constrained at the structural level.
In this case, final size is probably strongly constrained by the pot and may be largely independent of initial (i.e. seed) mass for example, Susko and Cavers [10] showed that individuals from large seeds were still larger after 15 days of growth in pots but that such differences disappeared at later dates.
Thus, the three dimensional conformation of the protein is most probably constrained by the number and hydrophobic nature of these tryptophan residues.
This is probably more about constraining cost increases rather than reducing costs, because the demand for information technology is still going up.
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