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Evidence suggests that inflammatory bowel disease-associated cancers arise from a larger field of colorectal mucosa that is "preconditioned" with a mutational burden that confers an increased propensity for further neoplastic progression, a phenomenon known as "field cancerization" (20, 21, 22).
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DGA columns were preconditioned with 3.0 M HCl.
The nanofibers mat was preconditioned with 200 μL methanol and 200 μL water once each.
Sample bottles were preconditioned with 5% nitric acid and rinsed with distilled deionized water.
The tC18 Sep-Pak cartridge was preconditioned with EtOH (5 mL) and sterile water (10 mL).
The QMA cartridge was preconditioned with 10 mL of 1 M potassium bicarbonate followed by 10 mL of 0.1-μm-filtered 18 MΩ water, and the silica cartridges were preconditioned with 10 mL of anhydrous hexane.
Strong cation exchange (SCX) cartridge was purchased from GRACE (Deerfield, IL, USA) and was preconditioned with 10 mL of water.
C18 Plus and tC18 Cartridges were preconditioned with 1 mL ethanol, and followed by rinse with 10 mL water.
The eluate was trapped on a cation exchange cartridge (BondElut SCX, Varian) which had been preconditioned with 5.5 M HCl.
The rat brain was preconditioned with 1 U thrombin by direct infusion into the left caudate nucleus.
SPE analysis was performed on Oasis cartridges (Waters Oasis® HLB, 30 μm, 60 mg) that were preconditioned with 1 ml ethanol followed by 5 ml water.
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