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These results suggested that EGFR is physically associated with CCN2, and this association is unaffected by the presence of EGFR native ligands, blocking antibody, or a known CCN2-binding protein, such as TrkA.
Of note, mTORC1 is physically associated with the mitochondria through the FKBP12-rapamycin associated protein (FRAP) (Desai, et al., 2002); however, there is little knowledge about the molecular mechanism involved in such interaction and its cellular effects during chronic mTORC1 stimulation.
We have also demonstrated that BRCA1 is physically associated with the promoter regions of basal genes through an association with c-myc.
Quantification of the statistical confidence that a protein pair is physically associated was performed using Fisher's exact test comparing the distribution of the association scores of that protein pair with the distribution of the association scores of the negative control measurements.
CD81 is physically associated with CD4 and CD8 and antibodies against CD81 generate signals which influence thymocyte adhesion and proliferation.
These data provide evidence that the E1B-58K antisen is physically associated with an E4-25K protein in productively infected cells.
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A large portion of keratins and keratin-associated proteins, most of which are physically associated in genomic clusters, behaved similarly, showing induction at 6 h following 0.2 Gy irradiation and repression or no modulation at the higher dose.
For example, paxillin was found to be physically associated with vinculin and FAK, however, no association was found between vinculin and FAK.
Calculations of the probability that the clusters represent chance superpositions indicate that the double clusters are physically associated.
Since DAXX is a key regulator of gene expression35,36 and has been shown to indirectly regulate PTEN stability37, we determined whether PTEN and DAXX could be physically associated.
This opposing force is termed normal force, which in contrast to gravity, is a contact force that acts upon objects that are physically associated with one another.
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