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The other is a blocking DNA (B2), which is partially complementary to a part of the OTA aptamer and partially complementary to a part of the DNAzyme.
The artificial sRNAs mimic the bacterial noncoding sRNAs which possess an antisense domain that is partially complementary to the targeted mRNA.
In respect of molecular beacon based aptasensor, FRET between Trp3a-1 labeled with FAM and CS-Trp3a-1 labeled with BHQ-1 black BHQ-1 blacker-1) whole is partially complementary with the aptamer was used to detect L-Trp.
Next, bio-S1 could in situ trigger hybridization chain reaction (HCR) to create a long nicked double helices analogous (dsDNA) in the present of ssDNA S2 and ssDNA S3 (S3 is partially complementary to the S2) to obtain the DNA supersandwich structure.
Each miRNA molecule is partially complementary to one or more mRNA transcripts, and functions to down-regulate gene expression by inhibiting protein translation or destabilizing target transcripts [ 2].
The BCL2L2 mRNA (encoding Bcl-w) contains a 3′ untranslated region (3′UTR) sequence that is partially complementary to miR-205, and the E2F6 mRNA has a 3′UTR recognized by miR-31.
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Two hairpin probes H1 and H2 which were partially complementary were employed to construct the label-free and enzyme-free DNA machine.
A specific aptamer against H5N1 was selected and two single-stranded DNAs (ssDNA1 and ssDNA2) were designed to be partially complementary to two ends of the aptamer.
The dye-labeled sequence was designed to be partially complementary and include base-pair mismatches to facilitate displacement by a more energetically favorable, fully complementary recognition motif embedded within a 98-mer displacer sequence.
The first twelve nucleotides at each 3′ end (3′-UCGUU/CUUCGUCC-5′) as well as the last eleven nucleotides at each 5′ end (5′-AGCAGUAGCAA-3′) are conserved between genome segments (Desselberger et al., 1980; Robertson, 1979) and are partially complementary to each other, which enables the single stranded RNA to form a "panhandle" structure (Cheong et al., 1999; Desselberger et al., 1980).
These aptasensors contained six DNA strands termed as Kana aptamer-catalysis strand (Kana apt-C), Kana inhibit strand (Kana inh), OTC aptamer-catalysis strand (OTC apt-C), OTC inhibit strand (OTC inh), hairpin structures H1 and H2 which were partially complementary.
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