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The mutant K159A, which is partially active in vitro, did not show detectable activity in vivo, indicating that the three active site mutants, K159A, S235A, and S259A, can be used as inactive controls in an in vivo experiment.
Therefore, in E. coli K-12 and EHEC, SdiA is partially active even in the absence of AHL.
The Rv3802 mutants of S175ΔE and S86ΔE were essentially inactive, while the S87ΔE mutant is partially active in both assays (Table 1).
The Lys132Arg mutant is partially active, and in this case, with the higher pKa and greater length of the Arg side chain, the positively charged guanidinium moiety could possibly participate directly (i.e. without the requirement for an intervening water molecule) in lowering the pKa of the substrate's p-hydroxyl group.
Thus, the IIS pathway is partially active in the absence of Lnk.
Data presented in Figure 3D showed that GNF-2 at 0.5 μM is partially active in inhibiting Bcr-Abl auto-phosphorylation and STAT5α phosphorylation.
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In parallel, T fully restored the complete sequence of copulation, DES was partially active and the specific activation of ERα or ERβ only resulted in a very low frequency of mount attempts in few subjects.
This sgk-1 gf) sgk-1 gf suppressed the effects of a rict-1 hypomutation fat accumulation, body suppresseddevelopmenthend partially suppreffectshe effects of a rict-1 null mutation on body size, suggesting thypomorph(gf) allons the SGK-1 kinase to be partially active in the absence ofatTORC2 accumulation
Stability tests upon bovine fibrinogen showed that the optimum pH for the proteolytic activity was pH 8.0, though the enzyme was partially active in the pH range of 5.0 10.0).
ER signaling is still active (partly due to the release of its inhibition by AKT), while the apoptosis and proliferation pathways are partially active.
In fact, other stimulation parameters might be even more effective than the 1 Hz stimulation, and the 25 Hz stimulation might have been partially active in the present study, possibly reducing the effect in the group comparison.
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