Individual nuclei were outlined by using DAPI fluorescence, and the nuclear fluorescence of Cy3 was quantified by using Zeiss KS400 image analysis software (Carl Zeiss, Inc., Oberkochen, Germany).
Within each image, six random areas were outlined by using the polygon function and individually analyzed.
Time usage changes in clinic patient flow after implementation of interventions is outlined by Figure 3. Use of PFA was an effective technique to identify areas within the care process for improvement, target interventions, and measure effectiveness of interventions.
Lesions were outlined by hand using tools from the FMRIB Software Library (Smith et al., 2004; Woolrich et al., 2009).
Much information about the roles of ABC transporters has been deduced using loss of function mutants isolated in genetic screens [ 20] and the pressing need for complementary biochemical studies of plant ABC transporters was outlined by François Lefèvre, using ABC sub-class G as an exemplar [ 24].
Mounted, dried sections were exposed in a phosphorimager cassette and the CA3 subregion was outlined by hand and quantified using Imagequant (GE healthcare).
Each of the four ROIs were outlined by a freehand-drawn shape using an image analysis system (Image Pro Plus, Media Cybernetics, Marlow, United Kingdom and Histometrix, Kinetic Imaging, Liverpool, United Kingdom) at objective ×2.5 magnification.
The different types of Multi-Agent programming languages that may be used was outlined by [32].
After 15 20 min subsequent to puncturing the skin, the SPT reaction wheal and flare was outlined by black ink and clear tape was used to transfer the outline from the skin to the results sheet by the trained research assistant or the principal investigator.
Numerous skills necessary to use the SDAT were outlined by the group, the most important being good communication skills, such as the ability to build a meaningful relationship with the patient before going ahead with the semi-structured interview, and having empathetic listening skills.
