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The two-round amplification kit is optimized to amplify low volumes and poor quality RNA for Affymetrix array analysis.
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The assay was optimized to amplify PRV DNA by incubation at 63 °C for 1 h.
The assay was optimized to amplify CSFV RNA by incubation at 63 °C for 50 min.
Primers were optimized to amplify cDNA but not genomic DNA and to generate a single PCR product.
For each pair of primers, PCR conditions were optimized to amplify a single band.
The assay was optimized to amplify Y. ruckeri at 63°C using a set of 4 or 5 primers.
Each pair of primers was optimized to amplify only the anticipated PCR products, which were verified by DNA sequencing and melting curve analysis.
Exon 1 was not analyzed as the PCR could not be optimized to amplify a specific product, despite multiple different primer sets, most likely because of the high GC content.
A generic primer, which recognizes the adaptor sequence, is used to amplify ligated DNA fragments and PCR conditions are optimized to preferentially amplify fragments in the 250-1000 bp size range.
Those "tricks" involve the feed algorithm, which is optimized to keep giving the user validation from friends.
To illustrate, the authors make the following conclusion in the paper: "the Kenyan MG muscle tendon unit is optimized to favor efficient storage and recoil of elastic energy".
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CEO of Professional Science Editing for Scientists @ prosciediting.com