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Compounding time is optimised at the minimum level.
The dynamic range is optimised at higher flip angles and with linear k-space sampling.
Recent phantom data suggest that although submerged polyp conspicuity is optimised at 700 HU, it remains good at 500 HU [ 19].
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Blast designs were optimised at open-pit mine to control vibration in view of long-term safety and stability of the underground openings.
The LAMP reaction was optimised at 63 °C for 1 h using bacterial genomic DNA as the template and the products were visualised under ultra-violet light and analysed by agarose gel electrophoresis.
A set of six LAMP primers was designed to amplify the target RNA by incubation with Bst DNA polymerase plus reverse transcriptase and the reaction was optimised at 65 °C for 60 min.
He found that conversion was optimised at precisely the critical temperature.
The temperature of incubation was optimised at 62 °C, 64 °C, and 68 °C.
The excitation wavelength was optimised at 260 nm [ 11] where EthBr is indirectly excited by energy transfer from the DNA.
Therefore the displacement incubation time was optimised at 5 min and this value was fixed to examine the effect of temperature on the displacement efficiency.
The system was optimised at 95°C 3 min; 95°C 10s 58°CC 30s and 45 cycles with a cycle threshold (Ct) value of 32 ± 2.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com