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For the high and medium confident results we apply the filtering used for the cow genome in the database on the tRNAscan-SE website: The high confident results we report are filtered by HMM Score 10.0, 2'Scorecore 5.0, and COVE Score 20 when the tRNA is found by both first time parsers and by COVE Score above 55 when the tRNA is only found by one.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com