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The analysis of these types of problems is often carried out using either time domain or frequency domain methods.
Thus, the fabrication of tissue scaffolds using natural biomaterials is often carried out using conventional techniques, where the efficiency in mass transport of nutrients and removal of waste products within the construct is compromised.
Discussions with international MR radiographers indicated that such development is often carried out using informal methods that are highly dependent on local conditions, that are rarely reported in the public domain and the validities of which are therefore not open to scrutiny; in addition, care pathways need to be specific to local healthcare needs and culture.
Insertion of a lateral fibular head transfixion wire is often carried out using anatomical landmarks and palpation to plan placement.
Exhaustive depolymerization of heparin is often carried out using a cocktail containing all three enzymes.
RT-PCR is often carried out using oligo-dT priming to generically reverse transcribe mRNA from the polyA tail.
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Nonetheless, district energy simulations are often carried out using a limited set of archetype buildings.
One thing is at least clear for now: few studies offer consistent results that are of value, and it is difficult to compare studies because they are often carried out using poorly characterized nanomaterials and arbitrary experimental conditions.
These assays are often carried out using multiple drug compounds with chemically diverse structural features, often in a 96 well-plate format for maximum time-efficiency, and are supported using rapid liquid chromatographic (LC) sample introduction with a tandem mass spectrometry (MS/MS) selected reaction monitoring (SRM) endpoint, taking around 6.5 h per plate.
Thermocycling was often carried out using the "touchdown" protocols with annealing temperatures ranging from 65°C to 45°C, (depending on the primer melting temperature) and a range of cycles from 30 to 45 were performed.
Although tests for HPV and MBV are often carried out using DNA from hepatopancreatic tissues, our experience has shown that they can also be detected in DNA extracts of pleopod samples from infected shrimp.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com