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When the donor-labeled strand departs from the surface after complete unwinding, the total fluorescence signal drops to the background level because the acceptor is not excited efficiently by the excitation laser at 532 nm.
Among the most important biological fluorophores, tryptophan is not excited in the 355 nm laser approach because tryptophan requires excitation at wavelengths below ~310 nm [ 6, p. 28 12], see Fig. 1 (a).
Polanski is not excited by cruelty anymore.
Van Exel is not excited about playing for Golden State.
She is going to college soon, and we're lucky to afford it, but she is not excited.
Neil Sroka, the communications director at Democracy for America, agreed, arguing that the party's base is not excited about adding a moderate, white man to the court.
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She's isn't excited by health reform.
"I'm not excited about my vote".
"I'm not excited," she said.
"People are not excited about getting involved.
"I'm not excited to do that.
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CEO of Professional Science Editing for Scientists @ prosciediting.com