The expression level of the target miRNA was normalized to expression of small nucleolar RNA 202 (sno202) and that of target mRNA was normalized to the expression of GAPDH.
Data were normalized to expression of Tbp.
Results were normalized to expression of 18sRNA host-gene RN18S1.
The detection was normalized to expression of the endogenous control, α-tubulin (Figure S8).
All quantitative RT-PCR results were normalized to expression of the β2-microglobulin gene.
Amounts of cDNA used in reactions were normalized to expression levels of GAPDH in each tissue.
Transcript abundance was normalized to expression of beta-actin within each sample, and relative expression was compared with that of whole thymus.
Data were normalized to expression levels of cpt-6, which encodes a carnitine palmitoyl transferase whose expression levels are unchanged by hypertonicity.
Values were normalized to expression of B6 fertile controls.
Data were normalized to expression levels of hypoxanthine phosphoribosyltransferase 1.
