ELISA signal was normalized to cell retention assessed by crystal violet staining, and signals were normalized to P66M (WT).
The mitochondrial respiration (OCR) was assessed using sequential injection of 1 μM (Huh7) or 2 μM (Hep3B) oligomycin, 0.2 μM carbonyl cyanide 4- trifluoromethoxy) phenylhydrazone and 2 μM antimycin A. Both ECAR and OCR measurements were normalized to cell number.
The caspase-3 activity was normalized to cell counts.
Values were normalized to cell numbers and to transfection efficiency.
The PGE2 concentration was normalized to cell protein.
Signals were normalized to cell number, which was estimated from measurements of FM1-43 fluorescence.
For each cell culture, the absolute fluorescence numbers were normalized to cell size.
No leak subtraction was used during current recording and current densities were normalized to cell capacitances.
The glucose concentration was normalized to cell density determined as protein content using Bradford protein assay.
