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PNAs are non-extendable oligonucleotides in which the ribose-phosphate backbone is replaced by 2-aminoethyl glycine units linked by amide bonds.
The behavior of the whole system can be treated as non-extendable for most applications, with a dead time of approximately 2.01 μs.
The superimposition and extension steps are repeated until a final non-extendable set is obtained.
The multiple alignment and extension steps are repeated until a final non-extendable set is obtained or upon completion of a predefined number of iterations.
Dual-fluorescent non-extendable probes were used and were labelled with 6-carboxyfluorescein at the 5′-end and with 6-carboxytetramethylrhodamine at the 3′-end in all primers and probe combinations (25 μl reaction volumes containing 900 nM primers and 250 nM Taqman® probe) and universal thermal cycling parameters.
A pseudo-fixed dead time correction circuit with non-extendable approximation is described that prevents systematic errors in quantitative EPMA caused by inappropriate dead time corrections.
While the dog is still learning good walk behavior, use a non-extendable leash between 4 and 6 feet long (1.2 1.8 meters), and avoid chokes, prong collars, and other collars that cause pain to the dog.
Given the likely steps involved in the telomerase pathway in yeast outlined above (see Figure 2), at least three plausible molecular mechanisms can be proposed to explain the switch at telomeres from extendable to non-extendable.
Although the effect of telomerase cellular concentration on the switch from non-extendable to extendable state at yeast telomeres has not been studied in detail, recent work (Mozdy et al, 2008) has shown that TLC1 template RNA levels limit telomere length and that over twenty genes affect TLC1 levels and average telomere length when mutated.
The non-extendable dead times used in most of the participations could be one of the causes responsible for the abnormal dispersion of the results.
Waist circumference was measured in centimeters at the topline of the hip bone using a non-extendable tape.
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