Another example of the limitation of LOFT is that if two targeting constructs are needed to insert the 5' and 3' LoxP sites, as in the case of floxing a large DNA fragment, then creating the allelic series in LOFT will accordingly require two constructs.
MIS-TKA demands an effort on the part of the surgeon [ 26], the learning curve may be unacceptably long for a low-volume arthroplasty surgeon [ 27], it takes more intervention length, require longer tourniquet time and special tools are needed to insert the implant.
However, further research into the genes related to CNVs and a comprehensive study on inserted CNVs is needed to form a more complete picture of the genetic structure variation in the bovine genome.
Since only portions of these genomes have been scanned, further studies are needed to determine the full insert length for many of the known Wolbachia homologs and to reveal more transferred sequences, some of which may encode full-length gene products.
This is particularly true for complex eukaryotic genomes, for which large quantities of DNA are needed to generate either large insert libraries or long sequence reads.
Further investigations are needed to determine the maximal insert size compatible with virus infectivity and systemic movement for a given genotype.
Without lost of generality, we assume (Omega ={omega _{1},omega _{2},dots,omega _{2^{n}}}), since if less valuations are needed to satisfy ϕ, others can be inserted with zero probability.
Clearly further studies, especially in vivo studies comparing the AngioDynamics Morpheus PICC and other similar catheters to conventional centrally inserted catheters, are needed to confirm their accuracy in patients, especially critically ill patients.
