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How much heat is needed for fusion?

In S. cerevisiae it has been shown that actin disassembly is an early subreaction of vacuole membrane fusion, whereas the formation of F-actin is needed for fusion and is likely catalyzed by components activated by Cdc42 at the membrane surface., In this yeast, overexpression of either constitutively active or dominant-negative Cdc42 resulted in vacuole fragmentation.

Deuterium-tritium (D-T) fusion thus requires the nuclei to have lower kinetic energy than is needed for the fusion of more highly charged, heavier nuclei.

In sporulating diploid S. cerevisiae cells only Sso1p, but not Sso2p, is needed for membrane fusion during prospore membrane formation.

Trypsin promotes viral entry and replication because IAV does not encode a viral hemagglutinin processing protease in its own genes, and post-transcriptional hemagglutinin cleavage by cellular trypsin-type proteases is needed for membrane fusion activity, virus entry into cells, and multiple cycles of viral replication [ 13– 15].

In contrast, the tetraspanin protein CD9 is needed for sperm oocyte fusions (Le Naour et al. 2000).

These experiments and controls strongly suggest that a functional, dephosphorylated Maf1 is needed for repression in the fusion context, and support the utility of the Maf1 Rpc160 fusion tool for identifying relevant factors involved in phosphoregulation.

It has been shown that once inside cells, early chlamydial protein synthesis is needed for inclusion remodeling to avoid fusion with lysosomes and for inclusion trafficking to the pericentriolar region of the host cell [31].

A greater quantity of bone graft is needed for multi-segment long posterolateral fusion success.

In light of the lipid mixing experiments, we suspected that sphingopeptides modulate the membrane, hence preventing receptor diffusion that is needed for the initiation of the fusion process.

Some charged lipid is needed for quantitative protein binding to the vesicles and consequently fusion.