Elevated highways, train viaducts, airplane and balloon corridors are layered above the streets to serve the city as its buildings increased in height.
Indeed, Rosen sees opportunities around the marketing and advertising services that can be layered above certain payment or transaction platforms.
And that's layered above the 1% and 20% of profits that the underlying hedge funds typically charge.
TE 65% sucrose (2.5 ml) and then 1 ml of TE 10% sucrose were layered above the lysate.
250µL mineral oil was layered above each well to prevent evaporation over the course of the experiment.
Fresh medium was layered above the agarose every 3 5 days.
In short, an equal volume of 0.9% NaCl was added to the blood samples, and the diluted blood was layered above a Ficoll-Paque PREMIUM solution.
Briefly, in a 1.5-mL Eppendorf tube, a 1-mL aliquot of exponential growth-phase bacterial culture was suspended above 400 μL of bromododecane, which itself was layered above 100 μL of metabolite extraction fluid.
Clarified lysates (5 ml) were layered above a 5 ml sucrose cushion (20 mM Tris HCl, 500 mM NH4Cl, 10 mM MgCl2, 0.5 mM EDTA, 6 mM β-mercaptoethanol, 37% sucrose; pH 7.5) and were spun for 22 hr at 37.2k rpm in a Ti 70.1 rotor.
Briefly, BM cells, harvested as above, were layered on a discontinuous (52, 64, and 72%) Percoll gradient (100%Percoll=9 1Percoll/10×0× PBS).
There are layers above any top-down model and layers below any bottom-up model.
