AGBL4, ROBO2, EYS and RYR3 appeared to have two insertions in distinct sites in the same tumour, though these could be insertions at a single rearrangement junction, since this is known to occur [ 28].
Errors were characterized by comparison to the known reference sequences (see methods), showing that the primary error mode was insertions, at 12%, followed by deletions, 2% and apparent mismatch errors at 1%.
Bell et al. predicted that there were insertions that occur at the position of LRR12 in hTLR2, LRR4 in hTLR10, and LRR12 in hTLR1 [32].
In three out of the 20 deletions there are small insertions at their breakpoints, and no extensive homology, therefore we suggest these have been mediated by NHEJ.
After expanding positive clones, genomic DNA was obtained from the selected clones and was tested for insertion at the Pmp22 locus by PCR (Supplementary Figure 1B).
If insertion is attempted at an angle without initial purchase, there is a possibility of mini-implant slipping during insertion.
After launch by a Delta 2925H-9.5, two flybys of Venus, and two flybys of Mercury, orbit insertion is accomplished at the third Mercury encounter.
An information scar of a single nucleotide "T" insertion is present at the junction.
In cortical bone, this screw nut is threaded at insertion, and can have considerable strength.
