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Under visual control, the catheter was inserted such that the inflated balloon was clearly located within the jugular vein.
The targeting vector was inserted such that exons 3 5 were deleted, supposedly inactivating the gene and inserting a LacZ cassette containing a strong splice acceptor.
During construction of the pRS31x plasmids (Sikorski and Hieter 1989), the authors had intended for the CEN6/ ARSH4 cassette to be inserted such that CEN6 would be closest to the bla gene, but CEN6 is instead closer to the HIS3 marker in both pRS313 and pRS413.
Consistent with this observation, in the one case in which it has been examined (large-conductance ion channels in the nuclear membranes in Purkinje neurons), the channels in the inner and outer membrane are inserted such that in each membrane they have the same orientation relative to the lumen (i.e., they "point" exactly as do the arrows in Figures 3, 4 and 5 ) [ 304 ].
All grafts were measured and inserted such that the articular surface of the graft edges was flush with the articular cartilage of the recipient site.
Segment "B" is inserted in such a way that the ends of the strut are connected to the midpoint of the cables in segment "A".
In order to investigate the intrinsic toxicity of different disease-associated expanded repeat RNA sequences, a set of constructs were generated in which a termination codon is inserted ahead of the repeat sequence such that the repeat tract is effectively shifted into the 3′UTR of the transcript in each case (Fig. 2F).
A tin (Sn) wire (0.25 mm diameter, Goodfellow Ltd). is inserted in the capillary such that one end is kept fixed at 1 mm from the powered electrode.
With this technique, a bivalent oligonucleotide linker is inserted between two interacting regions such that the chromatin interactions are captured without prior chromatin fragmentation and proximity-based ligation.
In all of those cases, the precursor is inserted into the ER membrane such that the functional domains involved in transcription are displayed on the cytosolic side of the membrane.
When inserting transcriptional terminator sequences care must be taken to control for changes in spacing between DNA regulatory elements and to take account of regulatory elements that may be inadvertently inserted, such as promoters of resistance genes, since these may be able to drive expression of neighbouring genes or divert activities from nearby enhancers.
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