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"Genetic" mutations crop up as new material is inserted at random.
The reason for analysing many primary transformants (T0) resides in the mechanism of integration itself: since the new DNA is inserted at random in the plant genome, plants with one to several integrated copies are obtained, and the multiple copies can be found in one or more chromosome locations.
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This violation phase consisted of the presentation of the same language stream previously heard, but non-words and rule-words were inserted at random positions.
However, to date they have utilized conventional transgene technology, where transgenes are inserted at random locations throughout the genome.
We checked whether the 17 ancestral introns were inserted at random or showed a regular pattern along the coding sequence.
The conclusion is that either introns have been inserted preferentially into proto-splice sites, or they have been inserted at random but preferentially fixed if the sequence context was a proto-splice site.
Previous studies of human Tau toxicity in Drosophila have used transgenic lines generated by P-element mediated transgenesis (Wittmann et al., 2001; Jackson et al., 2002; Nishimura et al., 2004; Steinhilb et al., 2007a; Steinhilb et al., 2007b; Chatterjee et al., 2009) where constructs containing human Tau cDNA are inserted at random into the Drosophila genome (Spradling and Rubin, 1982).
In case of the unordered model, the spacer is inserted at a random position, in the other cases it is always inserted in the beginning of s.
To determine whether the gene body methylation status in the transgene of line E is determined by the Ci-Trl promoter, we examined a parallel transgenic line, line F. Line F has the same transgene as line E, but it is inserted at a different genomic locus by random integration (genomic position KHC2 275935).
Normally during infection retroviral DNAs are inserted at multiple, almost random sites in the cellular DNA.
A random pentapeptide cassette was inserted at 23 different positions of TEM-1 using pentapeptide-scanning mutagenesis) [ 20].
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