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Our preliminary data show that this protein is, indeed, detectable in the peripheral blood of several MM patients, while it is not revealed in healthy controls (data not shown).
If there is indeed detectable adaptive evolution of tuberculosis within patients, what are the sources of selection?
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Using immunocytochemistry we first checked that in our neuronal models GSK-3β was indeed detectable within mitochondria.
In fact, both factors were indeed detectable at day 2 and 4 of differentiation, respectively, and decreased afterwards (Fig. 3).
To verify that telomere length changes were indeed detectable by TRF Southern blotting, we performed in vitro TRF shortening by DNase I nuclease treatment of zebrafish genomic DNA (Figure S5C, a), as compared with longer (high) and shorter (low) human telomeres (Figure S5C, b).
Significant Ube1-dependent NEDDylation was indeed detectable, concomitant with a drop in free ubiquitin levels.
Both Na+/K+ ATPase and GLAST were indeed detectable also in the P4 exosome fraction.
A reducible interaction of endogenous NEDD8 with HA UBE1 was indeed detectable, demonstrating that NEDD8 is in principle available to UBE1 and that under endogenous conditions low UBE1 levels are limiting to the reaction.
Thus, it is apparent following critical examination of enrichments in non-canonical TFBS motifs in ChIP-seq data that cofactor motifs signatures are indeed detectable and retrievable through rigorous screening as described in our study.
Using a mec1-4 temperature-sensitive allele that exhibits chromosome fragility without external replication stress, we showed that ssDNA was indeed detectable at the sites of eventual chromosome breakage, lending additional support to our hypothesis.
With FLAG-Rad51 expressed at a high level, we found that Plk1-mediated Rad51 phosphorylation was indeed detectable, at a lower level, after BRCA2 downregulation, and that WT Rad51 was transiently recruited to HU-stalled replication forks.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com