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Second, as it is impractical to validate the ability of each siRNA duplex to silence its target mRNA before carrying out the screen, targeting each gene four times enables us to prioritize hits for follow-up based on whether different siRNAs give rise to a specific phenotype.
In such studies, it is impractical to validate every possible tumor type.
Importantly, it is impractical to validate an analytical method before knowing which metabolite is of interest as a biomarker.
While it is impractical to validate by alternative methods the protein expression measurements for all 150+ ECM proteins observed in our proteomics experiments, we have included immunoblot data (for LTBP3, EGLN1 and CYR61) and immunohistochemical staining (LTBP3) that confirm the differential expression of these proteins (see Figure 2 figure supplement 1).
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It is impractical to charge for these individually.
Therefore, it is impractical to simulate all hundreds of SAGD realizations.
It is impractical to have two expressions depending on the reliability index β.
It is impractical to process this volume of information in the context of infrastructure asset management.
It is impractical to block all protons in this way.
Therefore, it is impractical to process large datasets.
Clearly, it is impractical to infect humans with Ad36 to improve diabetes.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com