Exact(3)
Phytase is immobilized through covalent bonding by the creation of GA links on the surface of APTES-modified nanofibers and also concentration gradient of enzyme solution makes phytase migrate and get trapped inside the 3D structure of the PAAm/starch nanofibers.
It should also be noted that Zhang et al. [67] have investigated for the very first time the development of SiNWs-FET sensor based on the carbohydrate-protein interaction where unmodified carbohydrate is immobilized through the formation of an oxime bonding.
Depending on the sensitivity, specificity, and limit of detection, the recognition element is immobilized through several surface techniques, including physical adsorption, chemical adsorption, covalent binding, and affinity-based interactions.
Similar(57)
Functionalization of the agarose support with the ligands also requires a more extensive chemical method of immobilization; for example, in Daniels et al., ion-exchange ligands were immobilized through first selectively activating aldehyde groups with periodate.
Mechanism analysis indicated that Cd II) was immobilized through complexation and precipitation.
Commercial baker's yeast cells were immobilized through adhesion on jute fabric using polyethylenimine.
For cDNA and long oligonucleotide sequences, probes produced ex situ are typically spotted onto a positively charged surface, such as poly-lysine or amino-silane, and are immobilized through UV cross-linking [13] [15].
The DNA was immobilized through biotin streptavidin interaction on a SA sensor chip, and the interaction reached to 300 Response Unit.
Finally, NOX was immobilized at alkaline pH values onto agarose activated with glyoxyl groups (Gx-ag) where the enzyme was immobilized through its lysine rich regions, resulting in very intense covalent attachments [ 43].
The NR1 C-terminal sequence C0-C1-C2 or mutated NR1 T879A was immobilized through covalent attachment to NHS-activated sepharose 4 fast flow (#17-0906-01; GE Healthcare, Barcelona, Spain) according to the manufacturer's instructions.
To obtain a population of CoA-binding aptamers that was less constrained by experimental design, a new affinity matrix was synthesized in which the CoA was immobilized through a disulfide linkage to the sulfhydryl on the pantotheine arm.
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