They are, however, cumbersome, time-consuming and in need of optimization and standardization [13], thus it remains restricted to a limited number of specialized laboratories.
The polyelectrolyte based capping layers are however cumbersome to deposit and alternative barrier layers offering enzymatic retention in the films as well as permeability to small molecules, for example the substrates of the embedded enzymes, are highly desired.
The SDS-PAGE-based method is, however, rather cumbersome for quantification of the cleavage, is less suitable for regular cellular samples than recombinant Atg4, and is certainly not adaptable for high throughput.
Such a process is, however, often cumbersome and time-consuming: busy clinical teams often do not have the time to assist with recruitment; the alternative path of writing to each patient (eg, within a general practice) to ask if she would agree to researchers accessing her medical records to assess suitability for a study is no less onerous.
However, manual review is still cumbersome.
However, this assay is still cumbersome, time consuming, and despite recent efforts (Hofman et al, 2011b) not standardised enough in its current form to be routinely applicable in clinical studies.
The aggregation of predictions from different in silico programs is however still a cumbersome task that requires a lot of human intervention and ad hoc solutions.
Traditional cell sorting systems, however, are still cumbersome and costly, which limit the full utilization of these systems in various biomedical research and clinical applications [ 41].
Considering that an atom can be added to a growing molecule through one, two, or more bonds (depending on its valence), the set of reaction rules is straightforward however cumbersome if one starts to consider all possible atoms types.
Regimens based on LV5FU2 are, however, time consuming, cumbersome and expensive (Ross et al, 1998).
