The first TCMn TCMn pair is highlighted with arrows.
The NKE is highlighted in a green box and ETS-like motifs are highlighted with black arrow.
Genes highlighted with ribosome footprint analysis are highlighted with red arrows (DDIT3 = CHOP; DDIT4 = REDD1; PPP1R15A = GADD34).
Control (a), flexion (b) and extension (c) FSUs, where the reduction of intracellular content (1) and extracellular matrix (2) are highlighted with white arrows.
The DNA binding sites are indicated with black triangles (R35, T44, R45, T46, T47, R49 and 65H) (Xu et al., 2001), the ligand binding sites are shown with grey triangles (216G, 219S and 223W) (van Aalten et al., 2001), and the newly-proposed amino acids essential for DNA binding activity of FadR protein are highlighted with dark arrows (W60, F74 and W75).
The seven known DNA-binding sites (R35, T44, R45, T46, T47, R49 and 65H) are highlighted with black triangles (Xu et al., 2001), the three known ligand-binding sites are shown with grey triangles (216G, 219S and 223W) (van Aalten et al., 2001), and the newly-proposed amino acids with indirect role for FadR-DNA interaction are highlighted with blue arrows (W60, F74 and W75) (Zhang et al., 2014).
The additional bases identified in our cDNAs are highlighted with red rectangles in Fig. 1 and their positions in the genome are indicated by black arrows in Fig. 2. In WormBase release WS170 a single agrin transcript of 1483 aa is predicted that shares 88% identity with our experimentally determined sequence.
