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The extraction is further repeated with distilled methanol until the A650 of a 1 10 dilution of the supernatant fluid is less than 0.5.
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The product can be used in one more round of PEAR directly, and the process can be further repeated.
Moreover, the PEAR products can be used in a next round of PEAR amplification directly without any treatment, and the process can be further repeated.
Analyses were further repeated with maternal socioeconomic status and smoking included as covariates as these factors are known to influence birth weight and placental weight and could impact upon childhood neurodevelopment.
Those determinations that yielded either borderline or statistically significant changes were further repeated to confirm the results.
Staining on different substrates has been further repeated with an anti-IgM antiserum, to account for isotype-specific ANEA in sera of PBC patients.
The same procedure was further repeated with different set sizes (0.5 to 10 million reads per sample) in order to test for the influence of total read counts on the statistics.
This procedure was further repeated twice to yield cells from two different isolations, which resulted in eight RNA samples (n = 8) for each of the three different cell culture conditions.
Flannery's credibility on issues of toxicity is further undercut by repeated misstatements or overstatements of what the science reveals.
It is further assumed that repeat proteins have evolved from gene duplication events and provide a source of variability for interactions with binding partners [31].
The photostability of HyPer5 is further documented by repeat array scanning without loss of detection.
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CEO of Professional Science Editing for Scientists @ prosciediting.com