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Then, the supernatant is further extracted and cleaned using a dSPE technique.
The supernatant (non-chromatin fraction) is collected and the pellet (chromatin fraction) is further extracted in IP0.1 buffer (20 mM HEPES of pH 7.6, 10% glycerol, 25 mM MgCl2, 0.1 mM EDTA, 0.2% NP-40, 0.1 M potassium acetate, 1 mM NaVO4 and 50 mM NaF).
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The KOH non-extractable residue was further extracted with acetic-nitric acids for 1 h at 100°C and the remaining pellet was defined as crystalline cellulose.
The residue was further extracted by repeating the above extraction procedure and the collected supernatant was combined with the first supernatant.
Following the final organic extraction, the remaining aqueous component was further extracted twice with water, and protein removed by precipitation with 3 1 acetonitrile (extract B).
The bone pellets that were left undigested after this first extraction of TP1 and TP2 were further extracted by adding 5 ml of EDTA buffer and following the same procedure as above.
The rhizomes were further extracted by refluxing with water in a commercial extraction facility.
The non-KOH-extractable residue defined as crude cellulose, was further extracted with acetic:nitric acids:water (8 1 2) for 1 h at 100°C, and the remaining pellet was defined as cellulose.
The crude extract was further extracted with diethyl ether.
The contours of the cross-sectional image are further extracted through image processing and segmentation.
ROI level amyloid measurements were further extracted based on the MarsBaR AAL atlas.
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