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The as-synthesized V2O5 samples are composed of discrete and uniform micro-flower, which is further assembled from several nanosheets.
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Using PE joining and gap-filling, a total of 93,998, 96,119, 83,946, 93,496 and 83,467 unigenes (≥200 bp) were further assembled from these contigs respectively (Additional file 3: Table S2; Sheet B in Additional file 4: Table S3 and Additional file 5: Figure S2).
These assembled contigs were further assembled with long reads from PacBio.
Filtered reads were assembled with Velvet (Zerbino and Birney 2008) and a k-mer range from 23 to 63. Contigs were further assembled using the Geneious assembler (Drummond et al. 2011).
The unigenes assembled from ESTs and unaligned Illumina reads, respectively, were further assembled using iAssembler.
Individual amplicons derived from this process were further assembled using CAP3 [ 41, 42].
The transcriptome data from the de novo and genome-assisted approaches were further assembled using PASA pipeline version 2.0.237 with BLAT as the aligner.
These must be further assembled to make the Z ring in bacteria.
The contigs and singletons were further assembled using Phrap program.
These contigs were further assembled into unigenes.
Contigs were further assembled using the following strategies.
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