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In the ChIP-Seq protocol, DNA is fragmented by sonication, producing fragments with a range of sizes that run as a broad band/smear on an agarose gel.
With this method, genomic DNA is fragmented by restriction enzymes, fragments are amplified by PCR, radiolabeled, incubated with nuclear proteins and the resulting DNA-protein complexes are separated by two-dimensional PAGE.
THE web connects over a billion people, but it is fragmented by language.
America's start-ups have 300m customers on their doorstep; Europe's market is fragmented by language and local rules, which limits prospects in ventures where Britons have a natural bent, such as retailing.
But that time is fragmented by meetings.
After cell lysis the DNA is fragmented by sonication.
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Briefly, 3 μg of each genomic DNA was fragmented by sonication, and fragments of 150 200 bp were purified.
The mitochondrial DNA was fragmented by nebulization and fragments from 500 to 700 bp were selected for sequencing.
The genomic DNA was fragmented by ultrasonication, and the DNA fragments were subjected to the whole-genome sequencing workflow of the Illumina HiSeq 2000 system.
Approximately 5 µg of sheep genomic DNA was fragmented by focused-ultrasonication to generate fragments less than 800 bp long (Covaris, Inc. Woburn, Massachusetts USA).
Subsequently, 200 ng of genomic DNA was fragmented by Covaris (Covaris Inc, Massachusetts, USA) to generate a mean fragment distribution of 550 bp.
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