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The protein mixture is first resolved by nonreducing gel electrophoresis to separate complexes by size, followed by excision of a narrow gel strip in the sample lane over the entire molecular weight range.
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We cannot move forward in our relations unless the complex problems that have piled up are first resolved.
In this regard, the fusion center follows a two-step approach, where the transmit ambiguities are first resolved and then localization is conducted: 1.
Some also insisted that for any current Israeli settlers to live in Palestine, the issue of ownership of their property must be first resolved.
Samples were first resolved by electrophoresis on SDS-PAGE (4 12% Bis-Tris gels) and then transferred to polyvinylidene fluoride membrane.
Amplification products were first resolved by gel electrophoresis, which confirmed the existence of antisense transcripts as well as assay specificity (Figure 3A and Supplementary information file, Figure S10).
This problem was first resolved by pointing out that low juvenile establishment or survival lent the iteroparous strategy a fitness advantage [ 6].
For Western blotting, the proteins were first resolved by NuPAGE 4-12% Bis-Tris gel (Invitrogen) and then transferred to a PVDF membrane.
Proteins in cell lysates was first resolved by SDS-PAGE, then transferred to nitrocellulose membrane and subsequently incubated with primary antibodies.
For immunoblotting, aliquot of this cell extract was first resolved in 10% SDS-PAGE gel, and the protein bands were then transferred to Millipore (Billerica, MA, USA) PVDF membranes.
Sizes of pooled PCR amplicons were first resolved using capillary electrophoresis (ABI 3730 DNA analyzer, Applied Biosystems) and then sized with GeneMapper 4.0 software (Applied Biosystems) with GeneScan 1200 LIZ Size Standard Applied BiosystemsStandard Applied Biosystems
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