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Al3Ti is first precipitated and functions as the nucleus of heterogeneous nucleation during solidification.
The protein is first precipitated in a stirred tank under gentle agitation followed by a period of vigorous agitation and then a final period of gentle agitation.
The brief method of synthesizing hydrophobic CdSe/ZnS QDs into water soluble is as in reference [16] with little modification: the hydrophobic CdSe/ZnS QDs is first precipitated with acetone and then redispersed in trichloromethane.
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Briefly, proteins were first precipitated with a 10% sodium tungstate (Na2WO4) (Aldrich, Milwaukee, WI) solution.
In the P-CF mode, the protein was first precipitated and then post-translationally solubilized by detergents.
In the reciprocal experiment, the chromatin was first precipitated with the HA antibody and eluted with HA peptide.
Briefly, total IgGs were first precipitated with 33% ammonium sulfate and the IgG fraction was dialyzed against PBS [33].
In a reverse experiment ARF6 mutants were first precipitated and the presence of GFP-A-RAF in the precipitate was demonstrated by immunoblotting with anti-GFP antibodies.
The Aβ42 oligomers were first precipitated with acetone to exclude toxicity of the SDS and HFIP from the oligomer preparation protocol of Barghorn et al, [10].
Soluble proteins in the supernatant were first precipitated in freezing (−20°C) acetone, and then resuspended in a rehydratation solution buffer [5 M urea, 2 M thiourea, 4% (w/v) CHAPS, 60 mM DTT, 0.2% (v/v) Bio-Lyte (Bio-rad)].
The cross-linked complex purified by size exclusion chromatography (pooled fractions corresponding to 120 160 kDa) was first precipitated with methanol/chloroform according to a reported procedure [44] in order to remove SDS and to increase the protein concentration.
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