Hoxd13 was expressed further posteriorly than Hoxd12 in splanchnic (visceral) mesoderm and endoderm at the cloacal level between stages 25 and 28 (Fig. 3C, D and Fig. 4).
At the same stage, Hoxd12 was detected in the tail bud and cloacal regions (Fig. 2C and Fig. 3C), and Hoxd13 was expressed further posteriorly in both of these domains (Fig. 2D and Fig. 3D).
PPP1R2P9 is not evolving neutrally and may thus be expressed, further supporting a functional role for this pseudogene.
As there is already evidence from EST that these four InR are expressed, further studies are needed to confirm if they are expressed in different tissues and/or life stages i.e. if they have different functions.
Cell line work showed a differential response to 17β -oestradiol depending on which ER subtype was expressed, further emphasizing the distinct biological roles of the α and β receptors.
Importantly, these outer cells, in which both FoxG1 and Ras-dva1 are expressed, further give rise to the diencephalon (supplementary material Fig. S1K5), but not to the telencephalon (G. Eagleson, personal communication; see also Eagleson et al., 1995; Eagleson and Harris, 1990; Eagleson et al., 1986).
Additional studies targeting specific tissues, such as muscle, in which dystonin is expressed, are needed to further elucidate the underlying pathophysiology of the dt disease.
Further, Sox10 is expressed in foetal brain in the rodent [24], [25].
As eGFP is expressed by protein biosynthesis, no further staining was necessary.
Further, Lrp4 is expressed on the cell surface of cultured cortical neurons (Tian et al., 2006).
