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When this phenomenon is predominant, the field is excluded using the function dark_max (Additional file 1b, line 11 in Editor\variables).
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Dead cells were excluded using PI.
Patients with inner ear anomalies were excluded using CT images.
Previously interrogated precursors were excluded using a dynamic exclusion duration of 60 s.
Dead cells were excluded using LIVE/DEAD Fixable Dead Cell Stain Kit (Life Technologies, Inc.).
Dead cells were excluded using the viability dye 7AAD (1%; Beckman Coulter).
Intact cells were gated based on FSC and SSC and dead cells were excluded using DAPI staining.
Notably, the typical drawback of metabolome analysis, namely, the omnipresent leakage of metabolites, was excluded using the 13C PEC approach.
Doublets were excluded on the basis of forward scatter properties and dead cells were excluded using LIVE/DEAD Fixable Aqua Dead Cell Stain (Life Technologies).
Precursors were filtered according to charge state (2 6 z) and monoisotopic peak assignment, and previously selected peaks were excluded using a dynamic window of 60 s with a mass error ±10 ppm.
Any trimmed reads not 10 nt in length were excluded using reformat.sh from BBTools (minlength = 10 maxlength = 10) and these sequences searched for 10-nt motifs enriched relative to the control with DREME50 (-k 10 -dna -norc).
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