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Assuming that the average molar mass of the 4.7 Mb E. coli chromosome is 650 g mol−1, pX1.0 is estimated to replicate at a copy number of 3 9 copies per E. coli chromosome.
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The absolute limit of detection (LOD), defined as the lowest concentration of wt sequence detectable in all the nine replicates, was estimated to be about 14 genome copies, corresponding approximately to 0.01% wt, while the same target was detected in only six replicates when 2.8 copies were present (Table 2).
Transcript levels were estimated from replicate microarray analysis of total RNA which was then processed by robust multi-array (RMA) quantile normalisation [38].
Biological variation should be estimated through replicate samples within treatments.
Bootstrap support was estimated from 1000 replicates to evaluate the reliability of internal branches.
Even if QTL variances close to zero were estimated in single replicates as an effect of a weak data structure, especially in DYD models for short pedigrees, these estimates were not excluded from the analysis in all investigated situations.
Ability to germinate was estimated with four replicates of 30 seeds, sown on two layers of Whatman No. 1 paper wetted with 7·5 mL distilled water in 90-mm-diameter Petri dishes.
Cover was estimated using 5 replicate, fixed 50×50 cm quadrats randomly located on the reef flat.
Bootstrap [ 78] branch support (BS) levels were estimated with 100 replicates (MaxTrees set to 100), 10 random additions each.
Error bars were estimated from three replicates for the FACS measurements and six replicates for the GFP levels.
To estimate support for the clades, non-parametric bootstraps were estimated with 1000 replicates.
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