Confocal imaging demonstrated that both EGF-Au and EGF-Au-PEG NPs were efficiently bound and internalised by MDA-MB-468 cells.
M. luteus, but not S. aureus, was efficiently bound and phagocytosed (in an eater-independent manner) in Kc167 cells (Fig. 3D, E).
While in XDH the two residues seem to determine which substrates are efficiently bound at the active site, in mAOX1 more factors determine the binding and conversion of substrates.
Error products—off-pathway, incorrectly assembled, or damaged SNARE complexes would not be efficiently bound by SMs.
It thus appears likely that KLK associates with the plasma membrane resulting in a high surface density, so that negatively charged protein modifications such as sialic acids can be efficiently bound by the positive charges on the CAMP.
Unlike canonical bacterial mRNAs, which first bind to the small 30S ribosomal subunit prior to joining of the 50S subunit, leaderless mRNAs can be efficiently bound and read by nondissociated 70S ribosomes (O'Donnell and Janssen 2002; Moll et al. 2004; Udagawa et al. 2004).
However, while the signals of TAp63 clearly increased, no signal of ΔNp63 changed using the approach of real-time PCR (with the specific primers as shown in Supplementary Table S2), indicating that the potential OCT4 binding sites in the promoter of ΔNp63 could not be efficiently bound by OCT4 in 293FT cells.
PLC independence may play a role in contributing protection from arthritis by a number of potential mechanisms such as conferring an inability to bind an athritogenic peptide that is only efficiently bound in the context of the PLC.
For pDCs, the smallest complexes were most efficiently bound, although also the larger complexes formed in high NaCl concentrations associated stronger with pDCs as compared to CD1c+ DCs.
Interestingly, we found that K48-linked ubiquitin chains are less efficiently bound by oligomeric p62 compared to linear and K63-linked chains, possibly because K48-linked ubiquitin chains disrupt the p62 oligomers more effectively.
