Sentence examples for is digested together with from inspiring English sources

Increasingly the food he eats is digested together with stories we tell.

The tissue is digested with collagenase overnight.

Once a positive clone was identified, the insert was digested overnight with EcoRI together with empty pBABE-puro plasmid.

The recombinant plasmid pAN7-1-Chi67-1 was constructed as follows: pAN7-1 was digested with HindIII, and then ligated together with the fragments gpdA, Chi67-1 ORF and trpC, successively (Fig. 1a) using a pEASY-Uni Seamless Cloning and Assembly Kit (TransGen Biotech, China).

The PstI digest was performed as follows: samples (DNA together with adapters) were digested with 5 U PstI (New England Biolabs, Ipswich, Massachusetts, United States) in 20 μl volume containing 1x NEB CutSmart buffer and incubated at 37 °C for 2 h followed by heat inactivation (65 °C for 30 min).

The products were digested with ClaI/EcoRI and ligated together with the EcoRI/XbaI fragment from pCS2+ TPC1–Myc (encompassing the five Myc tags) into the ClaI and XbaI sites of pCS2+.

The fragment was digested with EcoRI, blunted with T4 DNA Polymerase, and lastly, digested with SalI.

Samples were digested with digestion solution in fume hood.

The samples together with blank controls were digested with 0.90 ml concentrated nitric acid (Baker InstrAvantorzed; Avantor Performance Materials) and diluted to 10.0 ml with ultrapure water (18.2 MΩcm).

Subsequently, this fragment and pFvL118 were digested with BsrGI and ligated together, creating an HA→V5 cassette.

Both fragments were digested with NcoI and ligated together; in the resulting ~1.6-kb fragment there remained 11 bp between the transcription start site and the terminator of the rgsA gene.