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It is difficult to interrogate the underlying transcription landscape of individual neurons.
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Why? Babies are difficult to interrogate; we can only guess.
This bias leads to areas of the genome that are difficult to interrogate with HTS which may require higher coverage, multiple HTS applications or necessitates improvements in HTS technology to be able to be sequenced to a sufficient depth to detect variants.
Unfortunately, given proprieties surrounding the Newbler software it is difficult to further interrogate the cause of the above results as the intricacies of this assembly algorithm are unknown.
This is difficult to imagine.
That is difficult to prove.
This is difficult to deny.
This is difficult to do.
It is difficult to know.
This is difficult to measure.
Culture is difficult to change.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com