Not all the three schemes, MLST, FAFLP and MLVA could split the L. interrogans cluster based on geographic or ecological basis and thus it would be difficult to highlight its routes of spread in and out of Andaman or out of other main lands.
Unfortunately, other candidate genes are difficult to highlight in this panel of cell lines, as regions recurrently affected by copy number gains and/or losses always involved large DNA segments.
Once obesity is established, it is difficult to treat, highlighting the urgent need for successful strategies and policies to revert trends in weight gain, sedentary lifestyles and inadequate nutritional habits, especially in vulnerable youth populations [ 6– 8].
In a career that included serving as head of the land and property unit with the United Nations transitional administration in Timor-Leste, where he helped to resolve land disputes in the newly independent country, Gray says it is difficult to pinpoint a highlight.
The faster habituation of the white-confined group is difficult to interpret, and highlights the need for a clearer understanding of the interaction between motivational state and vertical exploratory behavior in zebrafish.
Although seriously ill hospital patients want to discuss end-of-life issues, their preferred decision making role varies and is difficult to predict [ 25], highlighting the need for active and regular assessment of patients' decision making needs.
In the short space provided here, it is difficult to do justice and highlight the superb features of this magnificent book.
Breast cancer outcome, including response to therapy, risk of metastasis and survival, is difficult to predict using currently available methods, highlighting the urgent need for more informative biomarkers.
They also highlight how it is difficult to classify chemically induced alterations as adverse or nonadverse effects, given the dynamic and complex mechanisms responsible for blastocyst implantation and placenta development in the maternal uterus.
