To determine if NP1 stimulates autophagy in differentiating osteoblasts MC3T3-E1 cells were differentiated in the presence of NP1-MNP (30 μg/mL), and protein was harvested at the indicated time points.
For a d, iWAT precursors from mice were differentiated in the presence of the differentiation media (DM, white bars), supplemented with rosiglitazone to drive beige differentiation (DM+rosiglitazone, brown bars; n=4) or treated with GW9508 instead of rosiglitazone (DM+GW9508, blue bars; n=5; see the Methods section).
To demonstrate the synergetic effects of apoA-1 and BMP4 on promoting the cardiac differentiation of ESCs, untransduced D3 ESCs were differentiated in the presence or absence of either of both of the above factors.
(g) Cells were differentiated in the presence of 1 μM DEX, and protein levels of the adipogenic markers aP2 and perilipin were assessed at days 0, 6 and 14 of differentiation by western blotting analysis.
For a e, iBAT precursors were differentiated in the presence of standard culture medium supplemented as follows: with 10% delipidated serum (DS, white bars; n=4); with DS plus insulin, triiodothyronine and ascorbic acid (DS+ITA, brown bars; n=5); or with DS plus 100 μM GW9508 (blue bars; n=4).
Even more important, they need to ensure their products are differentiated in the marketplace.
In system level simulations, MBSFN area and E-MBMS service reception area were differentiated in the same way as it is proposed in[29].
EPL cells were differentiated in the presence of SB431542 or PD169316 (Sigma-Aldrich) (Figure 6C).
Interestingly, in the data reported here, pro-asthmatic BMDCs from C3H mice were differentiated in the presence of GM-CSF.
Primary osteoclast precursors isolated from bone marrow were differentiated in the presence of M-CSF and RANKL [40].
