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Assuming that the reference genome is correctly assembled, we compare the contig order and orientation between the predicted assembly and the reference genome.
The ability of mT3 E. coli to secrete effectors demonstrates that the type 3 secretion apparatus is correctly assembled.
The above accuracy assessment is on the basis of the assumption that the reference genome is correctly assembled.
Overall, assuming that the reference genome is correctly assembled, the corrected accuracy rate of our method is 93.6% (including consistent connections and correctable relocations).
DOI: http://dx.doi.org/10.7554/eLife.03587.006 10.7554/eLiFigure87.007 Figure 2 figure supplement 3. S-layer is correctly assembled in Caulobacter mutants affected in capsule production.
AlaRS (Alanyl-tRNA synthetase) presents an example of the optimal scenario, whereby the protein is not found at all with few reads, but then pieces come together with the addition of more reads until the final protein is correctly assembled.
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With this procedure, all R108 LPs were detected in both assemblies from subsampled reads indicating that LP genes can be correctly assembled and discovered at equivalent read coverage.
While the nucleolus is known to function in vital cellular events, little is known about how its components are correctly assembled.
The great majority of Gag-GFP present in cell culture supernatants was shown to be correctly assembled into virus-like particles of the expected size and morphology consistent with immature HIV-1 particles.
An immune-staining of the endothelial specific tight junction protein ZO1 (Fig. 5b, c) revealed an increasing amount of punctuated fluorescence at intracellular regions along with the growth of cells, which confirmed that the tight junctions had been correctly assembled.
Don't you remember that I am the girl who lived to ride The Zipper -- you know, the ride that whirled me round and round, exhilarated by the uncertainty surrounding whether or not the ride had been correctly assembled by the traveling fair.
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