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Because the DOTA is conjugated through one of its carboxylates, the DOTA chelate covers only 7 coordination sites on the Eu3+ ion.
The library consisted of 182 peptide mixtures having the general sequence Y-A-x-x-x-x-x-S/T-x-x-x-x-A-G-K-K biotin), where X represents an equimolar mixture of the 17 proteogenic amino acid residues (excluding Ser, Thr, and CY-A-x-x-x-x-x-S/T-x-x-x-x-A-G-K-K biotinr and Thr, and biotin is conjugated through an aminohexanoic acid spacer to the C-terminal LY-A-x-x-x-x-x-S/T-x-x-x-x-A-G-K-K biotin
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In the complex, an A10 RNA aptamer targeting PSMA cells and a DUP-1 peptide aptamer specific to PSMA cells were conjugated through streptavidin.
The individual ubiquitin units of each chain are conjugated through an isopeptide bond between Lys48 and the C-terminal Gly76 of the preceding units.
Polylysine was conjugated through an N-terminal cysteine to a polyethylene glycol chain (PEG) by either a disulfide bond (SS) or a thioether bond (CS), and the resulting PEG peptide conjugates were used to compact plasmid DNA into reducible SS-DNA NPs or non-reducible CS-DNA NPs with identical physical properties.
Linear ubiquitin chains, which are conjugated through the amino-terminal methionine, also promote activation of the Nod2 signaling pathway by targeting Rip2.
CGP-58318 was conjugated through the primary amine group to Alexa Fluor 488 (AF488-BP) or Alexa Fluor 680 (AF680-BP) as previously described for alendronate (Thompson et al, 2006b).
ActA was conjugated through a heterobifunctional linker LC-SMCC (#22362; Thermo Scientific/Pierce, Rockford, IL), to poly-D-lysine (#P0296; Sigma Aldrich, St . Louis MO) labeled with ∼6 8 molecules of Bodipy FL-X-SE (#D6102; Molecular Probes) per peptide.
Similar to the previously reported ATP or GTP affinity probes, the SGTP affinity probe harbors a binding moiety (SGTP) and an enrichment moiety (i.e., desthiobiotin) that are conjugated through an acyl phosphate linkage.
The emerge of antibiotic resistant strains of Gram-positive bacteria that cause hospital-acquired diseases such as the methicillin-resistant Staphylococcus aureus (MRSA) has led to the identification of broad-host-range plasmids that carry antibiotic resistance and are conjugated through T4SSs.
First, the ubiquitin-like protein Atg8 is conjugated to phosphatidylethanolamine (PE) through the sequential actions of the E1-like enzyme Atg7 and the E2-like conjugating enzyme Atg3, while the second ubiquitin-like protein Atg12 is conjugated to Atg5 via the actions of Atg7 and the E2-like enzyme Atg10.
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