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Briefly, mouse brain DNA is cleaved with a restriction enzyme (Csp6I) and oligonucleotide linkers are added.
While the substrate is converted into a tetrahedral intermediate in the complex with RH-IN, the RT-RH peptide is cleaved, with both product peptides still bound in the active site.
Previous studies have shown that under these conditions GAD is cleaved with a pattern similar to that observed under excitotoxic conditions, particularly in the cerebellum and in the cerebral cortex [53].
ABI3 is cleaved with a perfect offset by 2 members of the grass specific miRNA family hvu-miR516.
The Y-shaped IgG is cleaved with pepsin to yield a V-shaped F ab' 2 or with papain to generate two Fab fragments.
In the nucleus, pri-miR is cleaved with RNase III Drosha to liberate the hairpin-shaped precursor miR that is transported from the nucleus with exportin-5 protein.
Similar(51)
Following the Me-RDA experimental design, Tester DNA was cleaved with FspBI and Driver DNA was cleaved with BfaI (see method, Figure 1A).
The resulting PCR-product was cleaved with NcoI- XbaI and ligated into the NcoI- XbaI-cleaved pBAD24 plasmid.
The GST tag was cleaved with PreScission protease at 4 °C overnight.
The His-tag (Tat) and His-GB1-tag (AFF) were cleaved with TEV protease.
Crosslinked DNA was cleaved with 1 M pyrrolidine (Sigma) at 90 °C for 20 min.
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