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To conclude, we show that each hydrophobic domain of TPCs is capable of insertion and forming oligomers, probably reflective of the ancestral one-repeat channel from which it evolved.
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This suggests that the AHLs used in this study were capable of insertion into the plasma membrane of Jurkat T-lymphocytes leading to changes in the dipole potential.
This study presents evidence that 3-oxo-C12 HSL and two close structural analogues, 3-oxo-C10-HSL and 3-oxo-C14-HSL are capable of insertion into the lipid bilayer in both artificial membrane and Jurkat T-lymphocyte cell systems.
Interestingly, pSLE1 contains two genes encoding putative integrases, suggesting that the plasmid might be capable of insertion into the chromosome.
As DNA transposons and retrotransposons have substantially different mechanisms of transposition [ 14], and given that much of the primary sequence of these regions has undergone significant change, it would seem unlikely that a molecular inhibition is in place that is capable of restricting insertion by such a wide range of mechanisms over such extended regions of sequence.
Our novel algorithm is capable of detecting insertions and deletions inaccessible by current methods.
This approach is extremely high-throughput, does not rely on cloning and is capable of detecting rare insertions.
We observed de novo assembly of soft-clipped and unmapped reads overcomes such limitation and is capable of detecting long insertions, even as long as 1 kb.
Our results suggest that ScanIndel is capable of predicting longer insertions with similar performance as the true assembly-based methods which tend to be most efficient in the detection of large insertions.
The preliminary data suggest that subtype C is capable of causing sequence insertions of longer length in the PTAP domain of the p6 gag, unlike other viral subtypes that insert sequences of shorter length at this location.
Our method has a distinctive feature that it is capable of judging whether an insertion occurs prior or posterior to the divergence of genomes.
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