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Protein content is calculated by duplicating a factor of 6.25 with N content.
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The expression data and associated technical errors on duplicates were calculated by using LightCycler Software 4.0 (Roche).
Antimicrobial susceptibility frequencies were calculated by using each of the 5 duplicate isolate removal methods described.
Synonymous and nonsynonymous substitution rates of duplicated genes were calculated by DnaSP v5.0 [ 63].
The number of nonsynonymous substitutions per nonsynonymous site (Ka) and the number of synonymous substitution per synonymous site (Ks) of duplicated genes were calculated by DnaSP 5.0 [ 25].
All tests were performed in biological triplicates and technical duplicates; p values were calculated by the Student's paired t-test with a two-tailed distribution: **p<0.01.
The frequency of IFN-γ-producing cells was calculated by averaging the number of spots in duplicate wells after background subtraction.
Antigen-specific responses were calculated by subtracting the average number of SFCs in duplicate donor-specific negative control wells from SFCs in test wells for each donor.
Experiments were performed in duplicates and percent viability was calculated by dividing the OD obtained in treatment group by OD of untreated cell control multiplied by hundred.
These fractions were titrated twice on MDBK monolayers in duplicate and viral titres were calculated by the Reed and Muench method [ 31].
Experiments were duplicated and the Kd value was calculated by Prism (SI-5).
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