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The kinase inhibitor cocktail used in this paper was adapted from previous work that showed that this inhibitor combination significantly improves specificity (reduce nonspecific phosphorylation by interfering kinases by 75%) and does not inhibit the kinase that is being assayed for.
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Here, the animals are being assayed for adult cuticle, so must have reach a particular stage.
This information can be used to determine which transcripts are being assayed, for example in the context of jointly analyzing in situ and microarray results.
Finally, we review the main therapeutic strategies that are being assayed to find a suitable treatment for HGPS.
For example, 1,10-phenanthroline is added to solutions that are assayed for iron II).
As all the pharmaceutical products are supposed to be assayed for potency, a validated TLC-densitometry method, demonstrating no interferences of degraded products with the drug active components can be useful in measuring these components in routine analysis.
Flow rates were calculated and the saliva was assayed for pH and for Na, K, Ca, Cl, inorganic P and protein concentrations.
Moreover, pMCV1.4-p31 pMCV1.4-p31ruct was assayed for molecular adjuvantcity's for a DNA vaccine against SVCV based in the surface antigen of this virus (pAE6-GSVCV).
Samples were centrifuged and supernatants were assayed for PGE2 content by ELISA.
All TIL cultures that yielded sufficient numbers of cells and for which appropriate target cells were available were assayed for tumor reactivity.
After 48 hours cells were harvested and cell lysates were assayed for luciferase activity.
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