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One feature I really liked is the ability to sync a particular folder to a corresponding folder on another drive to ensure that the original is always duplicated.
There is thus no reason now to expect a priori that DS of oriP is maximally efficient and is always duplicated in each cell cycle.
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Interestingly, we found that mutation in the first position of the repeat were not always duplicated in the following repeats contrary to that previously reported [40].
This allele was present in four individuals always as the most frequent one (Cluster1) (Additional file 2: Table S2), and the ratio in frequency compared to the second most frequent cluster (Cluster2) ranged in average from 1.9 to 3.9 fold for these individuals, reinforcing the hypothesis that this allele is always present in duplicate.
Presley was always "able to duplicate the open, hoarse, ecstatic, screaming, shouting, wailing, reckless sound of the black rhythm-and-blues and gospel singers," writes Pleasants, and also demonstrated a remarkable ability to assimilate many other vocal styles.
We therefore selected loci that were always present but not duplicated in any of the sequenced genomes.
Samples and standard curves were always processed in duplicates.
ELISA's were always performed in duplicate with the same samples applied to rtTA and control (293T cell lysate).
Every measure was always performed in duplicate (two PCR reactions from the same RT sample) and the average of those technical replicates was considered as the Ct for that particular sample.
Titrations were always performed in duplicates.
Samples were always processed in duplicate.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com