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The first reaction coil (6 ) enables binding of eluting ligands followed by a second incubation step in the second reaction coil (7 ) where the tracer ligand is allowed to bind to the remaining free receptor binding sites before detection by MS. Inset : schematic representation of the biochemical binding process during on-line bioaffinity assay with MS readout.
Then an enzyme labeled detector anti-cTnI antibody is allowed to bind with the immobilized cTnI.
We may instead supplement the language of propositional logic with a propositional quantifier, $\forall$, which is allowed to bind propositional letters such as $p$, $q$, $r$, ….
If more than one gold-labeled antibody is allowed to bind
In this mechanism, MK2 is allowed to bind ATF2 and prevent its interaction with p38.
[ 2] phospho-MK2 binds ATF2: phospho-MK2 is allowed to bind unphosphorylated ATF2 to form phospho-MK2 ATF2 phospho-MK2 ATF2k its interandion witherebyphosphorylation block8.
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The ligand binding assay was a variant of the ELISA in which dilutions of ligand (rhFGF-2) were allowed to bind to a ligand binding protein such as rhPln.D1 that had been coated onto the wells in PBS-N3.
The biotinylated peptides (20 pmol in 50 µl ddH2O) were allowed to bind the streptavidin-coated high-capacity binding plates (Pierce #15,503, Rockford, IL) overnight at 4°C or 2 hr at room temperature.
32P-labeled (1 pmol, 20,000 cpms) IDX-rasISS1 RNA was allowed to bind to 640 ng recombinant hnRNP H in binding buffer (1 mM ATP, 1.7 mM MgCl2 and 0.1 µM heparin in 1×Roeder D buffer).
After addition of a binding buffer and the magnetic particles (Siemens Healthcare Diagnostics, Cologne, Germany) nucleic acids were allowed to bind to the particles for 15 min at room temperature.
Then the mixtures were allowed to bind to glutathione-Sepharose.
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